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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IPO7 Monoclonal antibody

IPO7 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E7C12

Cat no : 67525-1-Ig

Synonyms

FLJ14581, Imp7, importin 7, IPO7, Ran binding protein 7, RANBP7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IPO7 Monoclonal antibody (67525-1-Ig)

    WB analysis using 67525-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67525-1-Ig (IPO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using IPO7 Monoclonal antibody (67525-1-Ig)

    WB analysis using 67525-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67525-1-Ig (IPO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IPO7 Monoclonal antibody (67525-1-Ig)

    IHC staining of human breast cancer using 67525-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67525-1-Ig (IPO7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IPO7 Monoclonal antibody (67525-1-Ig)

    IHC staining of human breast cancer using 67525-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67525-1-Ig (IPO7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, U2OS cells, LNCaP cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67525-1-Ig targets IPO7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IPO7 fusion protein Ag27257 相同性解析による交差性が予測される生物種
Full Name importin 7
Calculated molecular weight 120 kDa
Observed molecular weight 120 kDa
GenBank accession numberNM_006391.2
Gene symbol IPO7
Gene ID (NCBI) 10527
RRIDAB_2882745
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IPO7 antibody 67525-1-IgDownload protocol
IHC protocol for IPO7 antibody 67525-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols