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  • Featured Product
  • KD/KO Validated

IPPK Polyclonal antibody

IPPK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12603-1-AP

Synonyms

C9orf12, Ins(1,3,4,5,6)P5 2 kinase, InsP5 2 kinase, INSP5K2, IP5K, IPK1, IPK1 homolog, IPPK, KIAA0699

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using IPPK Polyclonal antibody (12603-1-AP)

    WB analysis of Jurkat using 12603-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12603-1-AP (IPPK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using IPPK Polyclonal antibody (12603-1-AP)

    IHC staining of human gliomas using 12603-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12603-1-AP (IPPK antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12603-1-AP targets IPPK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IPPK fusion protein Ag3278 相同性解析による交差性が予測される生物種
Full Name inositol 1,3,4,5,6-pentakisphosphate 2-kinase
Calculated molecular weight 491 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC026154
Gene symbol IPPK
Gene ID (NCBI) 64768
RRIDAB_2127848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IPPK antibody 12603-1-APDownload protocol
IHC protocol for IPPK antibody 12603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.

Authors - Paul C Scherer
  • KD Validated
View Article