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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IQCD Polyclonal antibody

IQCD Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26345-1-AP

Synonyms

4933433C09Rik, IQ domain containing protein D, IQ motif containing D, IQCD

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using IQCD Polyclonal antibody (26345-1-AP)

    WB analysis of mouse testis using 26345-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26345-1-AP (IQCD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IQCD Polyclonal antibody (26345-1-AP)

    IF Staining of HeLa using 26345-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26345-1-AP (IQCD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26345-1-AP is IQCD Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IQCD fusion protein Ag23692 相同性解析による交差性が予測される生物種
Full Name IQ motif containing D
Observed molecular weight 52 kDa
GenBank accession numberBC013151
Gene symbol IQCD
Gene ID (NCBI) 115811
RRIDAB_2880485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IQCD has some isoforms with MW 52 kDa, 37-40 kDa. All these isoforms can be detected by this antibody.

Protocols

Product Specific Protocols
WB protocol for IQCD antibody 26345-1-APDownload protocol
IF protocol for IQCD antibody 26345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Reprod Fertil Dev

Corrigendum to: IQ motif containing D (IQCD), a new acrosomal protein involved in the acrosome reaction and fertilisation.

Authors - Peng Zhang
View Article