Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 22167-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 22167-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 22167-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22167-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22167-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 22167-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 22167-1-AP
IP result of anti-IQGAP1 (IP:22167-1-AP, 4ug; Detection:22167-1-AP 1:8000) with HeLa cells lysate 1085 ug.
IHC staining of human breast cancer using 22167-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 22167-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 22167-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 22167-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 22167-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22167-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22167-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 22167-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 22167-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 22167-1-AP (IQGAP1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 22167-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of A549 using 22167-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of HeLa using 22167-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IQGAP1 (22167-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

IQGAP1 Polyclonal antibody

IQGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 22167-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Ras GTPase-activating-like protein IQGAP1, p195, KIAA0051, HUMORFA01

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, NIH/3T3 cells, SGC-7901 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, A549 cells, MCF-7 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 13 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

22167-1-AP targets IQGAP1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IQGAP1 fusion protein Ag17821 相同性解析による交差性が予測される生物種
Full Name	IQ motif containing GTPase activating protein 1
Calculated molecular weight	189 kDa
Observed molecular weight	190-195 kDa
GenBank accession number	BC151834
Gene Symbol	IQGAP1
Gene ID (NCBI)	8826
ENSEMBL Gene ID	ENSG00000140575
RRID	AB_10949193
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P46940
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

Protocols

Product Specific Protocols
WB protocol for IQGAP1 antibody 22167-1-AP	Download protocol
IHC protocol for IQGAP1 antibody 22167-1-AP	Download protocol
IF protocol for IQGAP1 antibody 22167-1-AP	Download protocol
IP protocol for IQGAP1 antibody 22167-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma Authors - Perla Pucci View Article
human	WB,IP	Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer Authors - Xingbin Hu KD Validated View Article
human	WB	Theranostics Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission. Authors - Hui-Fang Hu View Article
	WB	Oncogene The scaffold protein IQGAP1 links heat-induced stress signals to alternative splicing regulation in gastric cancer cells. Authors - Andrada-Maria Birladeanu KO Validated View Article
human	WB	Oncotarget Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1. Authors - Hyeongsun Moon View Article
human	WB,IF	Front Cell Infect Microbiol IQGAP1 Mediates Hcp1-Promoted Escherichia coli Meningitis by Stimulating the MAPK Pathway. Authors - Mingna Zhao KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22167-1-AP

WB analysis of NIH/3T3 using 22167-1-AP

WB analysis of SGC-7901 using 22167-1-AP

WB analysis of HeLa using 22167-1-AP

WB analysis of HEK-293 using 22167-1-AP

WB analysis of NIH/3T3 using 22167-1-AP

IP experiment of HeLa using 22167-1-AP

IHC staining of human breast cancer using 22167-1-AP

IHC staining of human breast cancer using 22167-1-AP

IHC staining of human colon using 22167-1-AP

IHC staining of human colon cancer using 22167-1-AP

IHC staining of human colon cancer using 22167-1-AP

IHC staining of human liver cancer using 22167-1-AP

IHC staining of human liver cancer using 22167-1-AP

IF Staining of A549 using 22167-1-AP

IF Staining of HeLa using 22167-1-AP

IF Staining of MCF-7 using 22167-1-AP

IF Staining of A549 using 22167-1-AP

FC experiment of HeLa using 22167-1-AP

IQGAP1 Polyclonal antibody

IQGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission.

The scaffold protein IQGAP1 links heat-induced stress signals to alternative splicing regulation in gastric cancer cells.

Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1.

IQGAP1 Mediates Hcp1-Promoted Escherichia coli Meningitis by Stimulating the MAPK Pathway.