Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HuH-7 cells, Ramos cells, human peripheral blood platelets, mouse testis tissue |
| Positive IHC detected in | human stomach tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30692-1-AP targets IQGAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IQGAP2 fusion protein Ag33518 相同性解析による交差性が予測される生物種 |
| Full Name | IQ motif containing GTPase activating protein 2 |
| Calculated molecular weight | 181 kDa |
| Observed molecular weight | 181 kDa |
| GenBank accession number | NM_006633 |
| Gene Symbol | IQGAP2 |
| Gene ID (NCBI) | 10788 |
| RRID | AB_3086388 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13576 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IQGAP2, a member of the IQGAP family, functions as a tumor suppressor in most of the cancers. IQGAP2 contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. IQGAP2 interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IQGAP2 antibody 30692-1-AP | Download protocol |
| IHC protocol for IQGAP2 antibody 30692-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |