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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse testis tissue using IQUB Polyclonal antibody (25605-1-AP)

    IP experiment of mouse testis using 25605-1-AP

    IP result of anti-IQUB (IP:25605-1-AP, 4ug; Detection:25605-1-AP 1:500) with mouse testis tissue lysate 1120 ug.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using IQUB Polyclonal antibody (25605-1-AP)

    IP experiment of mouse spleen using 25605-1-AP

    IP result of anti-IQUB (IP:25605-1-AP, 4ug; Detection:25605-1-AP 1:500) with mouse spleen tissue lysate 1920 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IQUB Polyclonal antibody (25605-1-AP)

    IHC staining of mouse testis using 25605-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25605-1-AP (IQUB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IQUB Polyclonal antibody (25605-1-AP)

    IHC staining of mouse testis using 25605-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25605-1-AP (IQUB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

IQUB Polyclonal antibody

IQUB Polyclonal Antibody for IHC, IP, ELISA
Cat No. 25605-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, ELISA

Synonyms

IQ motif and ubiquitin-like domain-containing protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inmouse spleen tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25605-1-AP targets IQUB in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IQUB fusion protein Ag19507 相同性解析による交差性が予測される生物種
Full Name IQ motif and ubiquitin domain containing
Calculated molecular weight 791 aa, 93 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC128181
Gene Symbol IQUB
Gene ID (NCBI) 154865
RRIDAB_3085807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NA54
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IQUB (IQ motif and ubiquitin domain containing) was discovered in 2002,4 which was located on chromosome 7q31.32 and its encoded protein mainly contained 2 domains, ubiquitin-like domain and IQ domain. IQUB had apparently higher expression in breast cancer than that in normal tissues, suggesting that it may act an important role in the occurrence and development of breast cancer (PMID: 29968965). IQUB is a critical RS1 adaptor in mouse sperm flagella, and is critical for sperm motility and fertilization (PMID: 36417862).

Protocols

Product Specific Protocols
IHC protocol for IQUB antibody 25605-1-APDownload protocol
IP protocol for IQUB antibody 25605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols