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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using IQUB Recombinant antibody (83847-5-RR)

    IHC staining of mouse testis using 83847-5-RR (same clone as 83847-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83847-5-RR (IQUB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83847-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IQUB Recombinant antibody (83847-5-RR)

    IHC staining of mouse testis using 83847-5-RR (same clone as 83847-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83847-5-RR (IQUB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83847-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83847-5-RR

    Affinity and Kinetic Characterization of 83847-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83847-5-RR against Human IQUB were performed. The affinity constant is 0.144 nM.

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IQUB Recombinant antibody, PBS Only

IQUB Uni-rAbTM Recombinant Antibody for IHC, ELISA
Cat No. 83847-5-PBS
Clone No.240957A11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

IQ motif and ubiquitin-like domain-containing protein, 240957A11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83847-5-PBS targets IQUB in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IQUB fusion protein Ag19507 相同性解析による交差性が予測される生物種
Full Name IQ motif and ubiquitin domain containing
Calculated molecular weight 791 aa, 93 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC128181
Gene Symbol IQUB
Gene ID (NCBI) 154865
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NA54
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IQUB (IQ motif and ubiquitin domain containing) was discovered in 2002,4 which was located on chromosome 7q31.32 and its encoded protein mainly contained 2 domains, ubiquitin-like domain and IQ domain. IQUB had apparently higher expression in breast cancer than that in normal tissues, suggesting that it may act an important role in the occurrence and development of breast cancer (PMID: 29968965). IQUB is a critical RS1 adaptor in mouse sperm flagella, and is critical for sperm motility and fertilization (PMID: 36417862).