- Featured Product
- KD/KO Validated
IRAK1 Monoclonal antibody, PBS Only
IRAK1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB, IHC, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
1H10A7
Cat no : 66653-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
66653-1-PBS targets IRAK1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | IRAK1 fusion protein Ag0728 相同性解析による交差性が予測される生物種 |
Full Name | interleukin-1 receptor-associated kinase 1 |
Calculated molecular weight | 77 kDa |
Observed molecular weight | 80 kDa |
GenBank accession number | BC014963 |
Gene symbol | IRAK1 |
Gene ID (NCBI) | 3654 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)