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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using IRAK1 Monoclonal antibody (66653-1-Ig)

    WB analysis of HEK-293 using 66653-1-Ig (same clone as 66653-1-PBS)

    WB result of IRAK1 antibody (66653-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using IRAK1 Monoclonal antibody (66653-1-Ig)

    WB analysis of HeLa using 66653-1-Ig (same clone as 66653-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using IRAK1 Monoclonal antibody (66653-1-Ig)

    WB analysis of HEK-293 using 66653-1-Ig (same clone as 66653-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using IRAK1 Monoclonal antibody (66653-1-Ig)

    WB analysis of MCF-7 using 66653-1-Ig (same clone as 66653-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IRAK1 Monoclonal antibody (66653-1-Ig)

    WB analysis of Jurkat using 66653-1-Ig (same clone as 66653-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IRAK1 Monoclonal antibody (66653-1-Ig)

    IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IRAK1 Monoclonal antibody (66653-1-Ig)

    IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

IRAK1 Monoclonal antibody, PBS Only

IRAK1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66653-1-PBS
Clone No.1H10A7

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

IRAK, IRAK 1, IRAK1, pelle

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66653-1-PBS targets IRAK1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IRAK1 fusion protein Ag0728 相同性解析による交差性が予測される生物種
Full Name interleukin-1 receptor-associated kinase 1
Calculated molecular weight 77 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC014963
Gene Symbol IRAK1
Gene ID (NCBI) 3654
RRIDAB_2882010
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51617
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)