IRAK4 Polyclonal antibody

Name: IRAK4 Polyclonal antibody
Brand: Proteintech
SKU: 18221-1-AP

IRAK4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18221-1-AP

Synonyms

IPD1, IRAK 4, IRAK4, NY REN 64, REN64

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of Jurkat using 18221-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 18221-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 18221-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 18221-1-AP
IP result of anti-IRAK4 (IP:18221-1-AP, 4ug; Detection:18221-1-AP 1:300) with K-562 cells lysate 4000ug.
IHC staining of human lung cancer using 18221-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18221-1-AP (IRAK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 18221-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18221-1-AP (IRAK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 18221-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 2 publications below

Product Information

The immunogen of 18221-1-AP is IRAK4 Fusion Protein expressed in E. coli.

Tested Reactivity	human
Cited Reactivity	human, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IRAK4 fusion protein Ag12982 相同性解析による交差性が予測される生物種
Full Name	interleukin-1 receptor-associated kinase 4
Calculated molecular weight	52 kDa
Observed molecular weight	52 kDa
GenBank accession number	BC013316
Gene symbol	IRAK4
Gene ID (NCBI)	51135
RRID	AB_2878519
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for IRAK4 antibody 18221-1-AP	Download protocol
IHC protocol for IRAK4 antibody 18221-1-AP	Download protocol
IP protocol for IRAK4 antibody 18221-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cell Rep Med Integrated characterization of hepatobiliary tumor organoids provides a potential landscape of pharmacogenomic interactions Authors - Yanjing Zhu View Article
	WB	Mol Neurobiol Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment Authors - Liyun Deng View Article
	WB	Front Pharmacol Euphorbia Factor L2 ameliorates the Progression of K/BxN Serum-Induced Arthritis by Blocking TLR7 Mediated IRAK4/IKKβ/IRF5 and NF-kB Signaling Pathways. Authors - Jing Tang View Article
sheep	WB	Mediators Inflamm Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells. Authors - Zhongjia Jiang View Article
	WB	Evid Based Complement Alternat Med Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-κB Activation. Authors - Xiangbing Shu View Article
human	WB	Int Immunopharmacol Herbal compound cepharanthine attenuates inflammatory arthritis by blocking macrophage M1 polarization Authors - Chenyang Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRAK4 Polyclonal antibody

IRAK4 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of Jurkat using 18221-1-AP

WB analysis of Jurkat using 18221-1-AP

WB analysis of K-562 using 18221-1-AP

IP experiment of K-562 using 18221-1-AP

IHC staining of human lung cancer using 18221-1-AP

IHC staining of human lung cancer using 18221-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Integrated characterization of hepatobiliary tumor organoids provides a potential landscape of pharmacogenomic interactions

Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment

Euphorbia Factor L2 ameliorates the Progression of K/BxN Serum-Induced Arthritis by Blocking TLR7 Mediated IRAK4/IKKβ/IRF5 and NF-kB Signaling Pathways.

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-κB Activation.

Herbal compound cepharanthine attenuates inflammatory arthritis by blocking macrophage M1 polarization