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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRE1; ERN1 Polyclonal antibody, PBS Only

IRE1; ERN1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27528-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IRE1; ERN1 Polyclonal antibody (27528-1-AP)

    WB analysis using 27528-1-AP (same clone as 27528-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27528-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using IRE1; ERN1 Polyclonal antibody (27528-1-AP)

    IP experiment of A549 using 27528-1-AP (same clone as 27528-1-PBS)

    IP result of anti-IRE1; ERN1 (IP:27528-1-AP, 4ug; Detection:27528-1-AP 1:500) with A549 cells lysate 600 ug. This data was developed using the same antibody clone with 27528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IRE1; ERN1 Polyclonal antibody (27528-1-AP)

    IHC staining of human pancreas cancer using 27528-1-AP (same clone as 27528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IRE1; ERN1 Polyclonal antibody (27528-1-AP)

    IHC staining of human pancreas cancer using 27528-1-AP (same clone as 27528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27528-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using IRE1; ERN1 Polyclonal antibody (27528-1-AP)

    FC experiment of A549 using 27528-1-AP (same clone as 27528-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human IRE1; ERN1 (27528-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27528-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27528-1-PBS targets IRE1; ERN1 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRE1; ERN1 fusion protein Ag25580 相同性解析による交差性が予測される生物種
Full Name endoplasmic reticulum to nucleus signaling 1
Calculated molecular weight 977 aa, 110 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC130405
Gene symbol IRE1
Gene ID (NCBI) 2081
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)