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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRE1; ERN1 Polyclonal antibody

IRE1; ERN1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28164-1-AP

Synonyms

ERN1, FLJ30999, hIRE1p, Inositol requiring protein 1, IRE1, Ire1 alpha, IRE1; ERN1, IRE1P

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IRE1; ERN1 Polyclonal antibody (28164-1-AP)

    WB analysis using 28164-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 28164-1-AP (IRE1; ERN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28164-1-AP targets IRE1; ERN1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRE1; ERN1 fusion protein Ag27375 相同性解析による交差性が予測される生物種
Full Name endoplasmic reticulum to nucleus signaling 1
Calculated molecular weight 977 aa, 110 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC130405
Gene symbol IRE1
Gene ID (NCBI) 2081
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)

Protocols

Product Specific Protocols
WB protocol for IRE1; ERN1 antibody 28164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols