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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IREB2 Polyclonal antibody

IREB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 29976-1-AP

Synonyms

IRP2, Iron-responsive element-binding protein 2, IREBP2, IRE-BP 2, IREB 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IREB2 Polyclonal antibody (29976-1-AP)

    WB analysis using 29976-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29976-1-AP (IREB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using IREB2 Polyclonal antibody (29976-1-AP)

    IP experiment of mouse liver using 29976-1-AP

    IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:5000) with mouse liver tissue lysate 1840 ug.

  • Immunoprecipitation (IP) experiment of A549 cells using IREB2 Polyclonal antibody (29976-1-AP)

    IP experiment of A549 using 29976-1-AP

    IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:1000) with A549 cells lysate 920 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IREB2 Polyclonal antibody (29976-1-AP)

    IHC staining of human colon cancer using 29976-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IREB2 Polyclonal antibody (29976-1-AP)

    IHC staining of human colon cancer using 29976-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using IREB2 Polyclonal antibody (29976-1-AP)

    IF Staining of A549 using 29976-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IREB2 antibody (29976-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inA549 cells, mouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29976-1-AP targets IREB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IREB2 fusion protein Ag32233 相同性解析による交差性が予測される生物種
Full Name iron-responsive element binding protein 2
Calculated molecular weight 105 kDa
Observed molecular weight105 kDa
GenBank accession numberBC017880
Gene symbol IREB2
Gene ID (NCBI) 3658
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.

Protocols

Product Specific Protocols
WB protocol for IREB2 antibody 29976-1-APDownload protocol
IHC protocol for IREB2 antibody 29976-1-APDownload protocol
IF protocol for IREB2 antibody 29976-1-APDownload protocol
IP protocol for IREB2 antibody 29976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols