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- KD/KO Validated
IRF2BP2 Polyclonal antibody
IRF2BP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 18847-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung squamous cell carcinoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 10 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
18847-1-AP targets IRF2BP2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | interferon regulatory factor 2 binding protein 2 |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | NM_182972 |
| Gene symbol | IRF2BP2 |
| Gene ID (NCBI) | 359948 |
| RRID | AB_10598163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| IHC protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| IF protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| IP protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Discov Unleashing Cell-Intrinsic Inflammation as a Strategy to Kill AML Blasts.
| ||
Hepatology The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop. | ||
Cell Death Dis SPOP point mutations regulate substrate preference and affect its function | ||
EMBO Rep Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling. | ||
Hypertension Control of Pathological Cardiac Hypertrophy by Transcriptional Corepressor IRF2BP2 (Interferon Regulatory Factor-2 Binding Protein 2).
|
