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  • Featured Product
  • KD/KO Validated

IRF3 Polyclonal antibody, PBS Only

IRF3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11312-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis of HeLa using 11312-1-AP (same clone as 11312-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis of Jurkat cells using 11312-1-AP (same clone as 11312-1-PBS)

    WB result of IRF3 specific antibody (11312-1-AP, 1:5000) with si-control and si-IRF3 transfected Jurkat cells. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis of U-937 using 11312-1-AP (same clone as 11312-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis of human brain using 11312-1-AP (same clone as 11312-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using IRF3 Polyclonal antibody (11312-1-AP)

    IP experiment of Jurkat using 11312-1-AP (same clone as 11312-1-PBS)

    IP result of anti-IRF3 (IP:11312-1-AP, 3ug; Detection:11312-1-AP 1:1000) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using IRF3 Polyclonal antibody (11312-1-AP)

    IHC staining of human spleen using 11312-1-AP (same clone as 11312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 11312-1-AP (IRF3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using IRF3 Polyclonal antibody (11312-1-AP)

    IHC staining of human spleen using 11312-1-AP (same clone as 11312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 11312-1-AP (IRF3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IRF3 Polyclonal antibody (11312-1-AP)

    IF Staining of HeLa using 11312-1-AP (same clone as 11312-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IRF3 antibody (11312-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11312-1-PBS targets IRF3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF3 fusion protein Ag1858 相同性解析による交差性が予測される生物種
Full Name interferon regulatory factor 3
Calculated molecular weight 47 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC009395
Gene symbol IRF3
Gene ID (NCBI) 3661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa.