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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRF3 Monoclonal antibody, PBS Only

IRF3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E6G8

Cat no : 66670-1-PBS

Synonyms

interferon regulatory factor 3, IRF 3, IRF3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IRF3 Monoclonal antibody (66670-1-Ig)

    WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRF3 Monoclonal antibody (66670-1-Ig)

    WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using IRF3 Monoclonal antibody (66670-1-Ig)

    IHC staining of human spleen using 66670-1-Ig (same clone as 66670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IRF3 Monoclonal antibody (66670-1-Ig)

    IHC staining of human tonsillitis using 66670-1-Ig (same clone as 66670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IRF3 Monoclonal antibody (66670-1-Ig)

    IHC staining of human tonsillitis using 66670-1-Ig (same clone as 66670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using IRF3 Monoclonal antibody (66670-1-Ig)

    IHC staining of human spleen using 66670-1-Ig (same clone as 66670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66670-1-PBS targets IRF3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IRF3 fusion protein Ag27223 相同性解析による交差性が予測される生物種
Full Name interferon regulatory factor 3
Calculated molecular weight 47 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC009395
Gene symbol IRF3
Gene ID (NCBI) 3661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.