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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRF6 Polyclonal antibody

IRF6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12928-1-AP

Synonyms

PIT, OFC6, LPS, IRF-6, IRF 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IRF6 Polyclonal antibody (12928-1-AP)

    WB analysis using 12928-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12928-1-AP (IRF6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using IRF6 Polyclonal antibody (12928-1-AP)

    WB analysis of A549 using 12928-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12928-1-AP (IRF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung tissue using IRF6 Polyclonal antibody (12928-1-AP)

    IHC staining of human lung using 12928-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IRF6 Polyclonal antibody (12928-1-AP)

    IHC staining of mouse kidney using 12928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using IRF6 Polyclonal antibody (12928-1-AP)

    IHC staining of human lung using 12928-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IRF6 Polyclonal antibody (12928-1-AP)

    IHC staining of mouse kidney using 12928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using IRF6 Polyclonal antibody (12928-1-AP)

    IHC staining of human stomach cancer using 12928-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using IRF6 Polyclonal antibody (12928-1-AP)

    IHC staining of human stomach cancer using 12928-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using IRF6 Polyclonal antibody (12928-1-AP)

    IF Staining of HaCaT using 12928-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using IRF6 antibody (12928-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA431 cells, A549 cells, HaCaT cells
Positive IHC detected inhuman lung tissue, mouse kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12928-1-AP targets IRF6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF6 fusion protein Ag3623 相同性解析による交差性が予測される生物種
Full Name interferon regulatory factor 6
Calculated molecular weight 467 aa, 53 kDa
Observed molecular weight53-60 kDa
GenBank accession numberBC014852
Gene symbol IRF6
Gene ID (NCBI) 3664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The interferon regulatory factor (IRF) family of genes directs the expression and activity of interferon and thus can strictly regulate innate immunity. Interferon regulatory factor 6 (IRF6) belongs to a family of nine transcription factors that share a highly conserved helix−turn−helix DNA-binding domain and a less conserved protein-binding domain. IRF6 regulates the balance between immature ectoderm differentiation and proliferation during craniofacial morphogenesis. IRF6 functions as a tumor suppressor gene in breast cancer, cervical cancer, nasopharyngeal carcinoma and so on.

Protocols

Product Specific Protocols
WB protocol for IRF6 antibody 12928-1-APDownload protocol
IHC protocol for IRF6 antibody 12928-1-APDownload protocol
IF protocol for IRF6 antibody 12928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols