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IRS1 Polyclonal antibody

Name: IRS1 Polyclonal antibody
Brand: Proteintech
SKU: 17509-1-AP

IRS1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Dot blot, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17509-1-AP

Synonyms

IRS-1, IRS 1, insulin receptor substrate 1, HIRS 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of A549 using 17509-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis using 17509-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17509-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 17509-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 17509-1-AP
IP result of anti-IRS1 (IP:17509-1-AP, 5ug; Detection:17509-1-AP 1:1000) with A549 cells lysate 3500ug.
IHC staining of human breast cancer using 17509-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human breast cancer using 17509-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 17509-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 17509-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of A549 using 17509-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using IRS1 antibody (17509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of A549 using 17509-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using IRS1 antibody (17509-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of MCF-7 using 17509-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human IRS1 (17509-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 17509-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, A431 cells, HEK-293 cells, PC-3 cells
Positive IP detected in	A549 cells
Positive IHC detected in	human breast cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 66 publications below
IHC	See 7 publications below
IF	See 4 publications below
IP	See 2 publications below

Product Information

17509-1-AP targets IRS1 in WB, IHC, IF/ICC, FC (Intra), IP, Dot blot, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, pig, megalobrama amblycephala
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IRS1 fusion protein Ag11714 相同性解析による交差性が予測される生物種
Full Name	insulin receptor substrate 1
Calculated molecular weight	1242 aa, 132 kDa
Observed molecular weight	160-185 kDa
GenBank accession number	BC053895
Gene symbol	IRS1
Gene ID (NCBI)	3667
RRID	AB_10596914
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.

Protocols

Product Specific Protocols
WB protocol for IRS1 antibody 17509-1-AP	Download protocol
IHC protocol for IRS1 antibody 17509-1-AP	Download protocol
IF protocol for IRS1 antibody 17509-1-AP	Download protocol
IP protocol for IRS1 antibody 17509-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
pig	WB	Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation Authors - Xiaolei Lin View Article
human	WB	Mol Cell KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis Authors - Hiroshi Senoo View Article
mouse	WB	Autophagy BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation. Authors - Simone C da Silva Rosa View Article
mouse	Dot blot	Environ Health Perspect Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice. Authors - Jacob Raber View Article
mouse	WB	JHEP Rep Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis Authors - Fuji Yang View Article
human	WB	Clin Transl Med Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma. Authors - An-Dong Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRS1 Polyclonal antibody

IRS1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of A549 using 17509-1-AP

WB analysis using 17509-1-AP

WB analysis of HEK-293 using 17509-1-AP

WB analysis of A431 using 17509-1-AP

IP experiment of A549 using 17509-1-AP

IHC staining of human breast cancer using 17509-1-AP

IHC staining of human breast cancer using 17509-1-AP

IHC staining of human liver using 17509-1-AP

IHC staining of human liver using 17509-1-AP

IF Staining of A549 using 17509-1-AP

IF Staining of A549 using 17509-1-AP

FC experiment of MCF-7 using 17509-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Augmentation of scleral glycolysis promotes myopia through histone lactylation

KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis

BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.

Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.

Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.