Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | human colon cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24440-1-AP targets IRX6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IRX6 fusion protein Ag19623 相同性解析による交差性が予測される生物種 |
| Full Name | iroquois homeobox 6 |
| Calculated molecular weight | 446 aa, 48 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC136573 |
| Gene Symbol | IRX6 |
| Gene ID (NCBI) | 79190 |
| RRID | AB_2918082 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78412 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IRX6 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IRX6 antibody 24440-1-AP | Download protocol |
| IHC protocol for IRX6 antibody 24440-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |