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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ISG15 Monoclonal antibody, PBS Only

ISG15 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G9A2

Cat no : 68408-1-PBS

Synonyms

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ISG15 Monoclonal antibody (68408-1-Ig)

    WB analysis using 68408-1-Ig (same clone as 68408-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ISG15 Monoclonal antibody (68408-1-Ig)

    WB analysis of A431 using 68408-1-Ig (same clone as 68408-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ISG15 Monoclonal antibody (68408-1-Ig)

    WB analysis using 68408-1-Ig (same clone as 68408-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68408-1-PBS targets ISG15 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ISG15 fusion protein Ag8953 相同性解析による交差性が予測される生物種
Full Name ISG15 ubiquitin-like modifier
Calculated molecular weight 165 aa, 18 kDa
Observed molecular weight 13-17 kDa
GenBank accession numberBC009507
Gene symbol ISG15
Gene ID (NCBI) 9636
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.