Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse retina tissue, NIH/3T3 cells |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83782-5-RR targets ISLR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ISLR fusion protein Ag19446 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin superfamily containing leucine-rich repeat |
| Calculated molecular weight | 428 aa, 46 kDa |
| Observed molecular weight | 44-46 kDa |
| GenBank accession number | BC022478 |
| Gene Symbol | ISLR |
| Gene ID (NCBI) | 3671 |
| RRID | AB_3671374 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O14498 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Immunoglobulin superfamily containing leucine-rich repeat protein(ISLR), is involved in various biological events, such as embryonic development, Gaucher's disease, and replicative senescence of fibroblasts in some organs, including the heart, pancreas, and bone marrow. ISLR, also known as meflin, is a newly discovered marker of mesenchymal stem cells, which is involved in pathological fibrosis and the cancer microenvironment. (PMID: 34713300)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ISLR antibody 83782-5-RR | Download protocol |
| IHC protocol for ISLR antibody 83782-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |