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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse retina tissue using ISLR Recombinant antibody (83782-5-RR)

    WB analysis of mouse retina using 83782-5-RR

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 83782-5-RR (ISLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ISLR Recombinant antibody (83782-5-RR)

    WB analysis using 83782-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83782-5-RR (ISLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using ISLR Recombinant antibody (83782-5-RR)

    IHC staining of mouse eye using 83782-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83782-5-RR (ISLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83782-5-RR

    Affinity and Kinetic Characterization of 83782-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83782-5-RR against Human ISLR were performed. The affinity constant is 0.369 nM.

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ISLR Recombinant antibody

ISLR Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83782-5-RR
Clone No.240727D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

UNQ189/PRO215, Immunoglobulin superfamily containing leucine-rich repeat protein, HsT17563, 240727D4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse retina tissue, NIH/3T3 cells
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83782-5-RR targets ISLR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ISLR fusion protein Ag19446 相同性解析による交差性が予測される生物種
Full Name immunoglobulin superfamily containing leucine-rich repeat
Calculated molecular weight 428 aa, 46 kDa
Observed molecular weight44-46 kDa
GenBank accession numberBC022478
Gene Symbol ISLR
Gene ID (NCBI) 3671
RRIDAB_3671374
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO14498
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Immunoglobulin superfamily containing leucine-rich repeat protein(ISLR), is involved in various biological events, such as embryonic development, Gaucher's disease, and replicative senescence of fibroblasts in some organs, including the heart, pancreas, and bone marrow. ISLR, also known as meflin, is a newly discovered marker of mesenchymal stem cells, which is involved in pathological fibrosis and the cancer microenvironment. (PMID: 34713300)

Protocols

Product Specific Protocols
WB protocol for ISLR antibody 83782-5-RRDownload protocol
IHC protocol for ISLR antibody 83782-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols