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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ISLR2 Polyclonal antibody (24814-1-AP)

    WB analysis using 24814-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24814-1-AP (ISLR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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ISLR2 Polyclonal antibody

ISLR2 Polyclonal Antibody for WB, ELISA
Cat No. 24814-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

ISLR2, KIAA1465, LINX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24814-1-AP targets ISLR2 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISLR2 fusion protein Ag20518 相同性解析による交差性が予測される生物種
Full Name immunoglobulin superfamily containing leucine-rich repeat 2
Calculated molecular weight 745 aa, 79 kDa
Observed molecular weight79 kDa, 130 kDa
GenBank accession numberBC152429
Gene Symbol ISLR2
Gene ID (NCBI) 57611
RRIDAB_3669451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXK2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ISLR2 (immunoglobulin superfamily containing leucine rich repeat 2), also known as LINX. It is expected to be located in cell membrane and the protein is mainly expressed in brain and testis. While the first knockout mouse had impaired development of the internal capsule and no hydrocephalus, a much more recent independent knockout mouse was shown to additionally have severe hydrocephalus. Although the mechanism remains unclear, it may be related to the role of ISLR2 in reorganizing the cytoskeleton of developing neurons (PMID: 30483960). The calculated molecular weight of ISLR2 is 79 kDa, this target has phosphorylation modification and glycosylation modification. And the 130 kDa band is glycosylated (PMID: 29739947).

Protocols

Product Specific Protocols
WB protocol for ISLR2 antibody 24814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols