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KD/KO Validated

ITCH Polyclonal antibody

Name: ITCH Polyclonal antibody
Brand: Proteintech
SKU: 20920-1-AP
Rating: 5.00 (1 reviews)

ITCH Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 20920-1-AP

Synonyms

AIF4, AIP4, ITCH, NAPP1, NFE2 associated polypeptide 1

Validation Data Gallery

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AllWBIPIHC

WB analysis using 20920-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 20920-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 20920-1-AP
U-251 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 20920-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20920-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 20920-1-AP
HeLa lysates prepared and IP of ITCH performed using 2.0 μg of 20920-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of HEK-293 using 20920-1-AP
IP result of anti-ITCH (IP:20920-1-AP, 4ug; Detection:20920-1-AP 1:300) with HEK-293 cells lysate 2400 ug.
IHC staining of human breast cancer using 20920-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20920-1-AP (ITCH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 20920-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20920-1-AP (ITCH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 20920-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, U-251 cells, NIH/3T3 cells, K-562 cells, Hela cells, Raji cells
Positive IP detected in	HEK-293 cells, HeLa cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 13 publications below
IHC	See 1 publications below
IP	See 2 publications below
CoIP	See 3 publications below

Product Information

20920-1-AP targets ITCH in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ITCH fusion protein Ag15120 相同性解析による交差性が予測される生物種
Full Name	itchy E3 ubiquitin protein ligase homolog (mouse)
Calculated molecular weight	903 aa, 103 kDa
Observed molecular weight	103 kDa, 70 kDa
GenBank accession number	BC011571
Gene symbol	ITCH
Gene ID (NCBI)	83737
RRID	AB_2878765
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ITCH is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. It is involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. ITCH can be cleaved by caspase-3, -6, and -7 on residue Asp240 and form a 65 kDa cleaved form (PMID: 19541840).

Protocols

Product Specific Protocols
WB protocol for ITCH antibody 20920-1-AP	Download protocol
IHC protocol for ITCH antibody 20920-1-AP	Download protocol
IP protocol for ITCH antibody 20920-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol Heat stress activates YAP/TAZ to induce the heat shock transcriptome. Authors - Min Luo KD Validated View Article
human	WB	Dev Cell ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia. Authors - Jie Ran KD Validated View Article
human	WB	Theranostics ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3. Authors - Lijie Zhou View Article
human	WB,IP	Clin Transl Med GRAMD4 inhibits tumour metastasis by recruiting the E3 ligase ITCH to target TAK1 for degradation in hepatocellular carcinoma. Authors - Qian Yun Ge KD Validated View Article
human	WB, CoIP	Front Oncol Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation. Authors - Cong Wang KD Validated View Article
human	WB	J Mol Med (Berl) Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology. Authors - Yuhong Zhang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Chrisovalantou (Verified Customer) (10-31-2024)

Antibody was able to detech endogenous ITCH in SH-SY5Y and HEK293T on western blot

Applications: Western Blot
Primary Antibody Dilution: 1:1000
Cell Tissue Type: SH-SY5Y, HEK293T

ITCH Antibody Western Blot validation (1:1000 dilution) in SH-SY5Y, HEK293T (Cat no:20920-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ITCH Polyclonal antibody

ITCH Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 20920-1-AP

WB analysis of K-562 using 20920-1-AP

WB analysis of U-251 using 20920-1-AP

WB analysis of NIH/3T3 using 20920-1-AP

WB analysis of HEK-293 using 20920-1-AP

IP experiment of HeLa using 20920-1-AP

IP experiment of HEK-293 using 20920-1-AP

IHC staining of human breast cancer using 20920-1-AP

IHC staining of human breast cancer using 20920-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3.

GRAMD4 inhibits tumour metastasis by recruiting the E3 ligase ITCH to target TAK1 for degradation in hepatocellular carcinoma.

Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation.

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Reviews