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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ITCH Monoclonal antibody (67757-1-Ig)

    WB analysis using 67757-1-Ig (same clone as 67757-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using ITCH Monoclonal antibody (67757-1-Ig)

    WB analysis of K-562 using 67757-1-Ig (same clone as 67757-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ITCH Monoclonal antibody (67757-1-Ig)

    WB analysis of HSC-T6 using 67757-1-Ig (same clone as 67757-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using ITCH Monoclonal antibody (67757-1-Ig)

    WB analysis of NIH/3T3 using 67757-1-Ig (same clone as 67757-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using ITCH Monoclonal antibody (67757-1-Ig)

    WB analysis of LNCaP using 67757-1-Ig (same clone as 67757-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ITCH Monoclonal antibody (67757-1-Ig)

    IF Staining of HepG2 using 67757-1-Ig (same clone as 67757-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ITCH antibody (67757-1-Ig, Clone: 2E3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

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ITCH Monoclonal antibody, PBS Only

ITCH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67757-1-PBS
Clone No.2E3G9

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67757-1-PBS targets ITCH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ITCH fusion protein Ag14688 相同性解析による交差性が予測される生物種
Full Name itchy E3 ubiquitin protein ligase homolog (mouse)
Calculated molecular weight 903 aa, 103 kDa
Observed molecular weight103 kDa, 70 kDa
GenBank accession numberBC011571
Gene Symbol ITCH
Gene ID (NCBI) 83737
RRIDAB_2918525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96J02
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.