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  • KD/KO Validated

Integrin alpha 1 Polyclonal antibody

Integrin alpha 1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Cat no : 22146-1-AP

Synonyms

CD49a, Integrin alpha 1, integrin, alpha 1, ITGA1, VLA 1, VLA1


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Tested Applications

Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22146-1-AP targets Integrin alpha 1 in WB, IHC, ELISA, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin alpha 1 fusion protein Ag17584 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 1
Calculated molecular weight 1179 aa, 131 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC137121
Gene symbol Integrin alpha 1
Gene ID (NCBI) 3672
RRIDAB_2879008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha 1 antibody 22146-1-APDownload protocol
IHC protocol for Integrin alpha 1 antibody 22146-1-APDownload protocol
FC protocol for Integrin alpha 1 antibody 22146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer.

Authors - Hai Li
  • KD Validated
humanWB

Int J Oncol

Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules

Authors - AMINA BOLATKAN
humanWB

J Ethnopharmacol

A combination index and glycoproteomics-based approach revealed synergistic anticancer effects of curcuminoids of turmeric against prostate cancer PC3 cells.

Authors - Meng-Meng Wei
humanWB,IHC,IF

Int J Oncol

FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.

Authors - Junfen Xu
ratWB,IF

J Biomech

Integrin endocytosis on elastic substrates mediates mechanosensing.

Authors - Jing Li
ratWB

Med Sci Monit

17β-Estradiol Protects Rat Annulus Fibrosus Cells Against Apoptosis via α1 Integrin-Mediated Adhesion to Type I Collagen: An In-vitro Study.

Authors - Chun-Ming Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (04-24-2019)

This antibody labelled a band above 250 kD, which is likely the glycosylated form of Itga1. But, it did not label any band around 170 kD as shown in the data sheet.

  • Applications: Western Blot,
  • Cell Tissue Type: MEF