Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 10569-1-AP
WB result of Integrin alpha-5 antibody (10569-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin alpha-5 transfected HeLa cells.
WB analysis using 10569-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10569-1-AP (Integrin Alpha-5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10569-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10569-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10569-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COS-7 using 10569-1-AP
COS-7 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of 3T3-L1 using 10569-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human skin cancer using 10569-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10569-1-AP (Integrin Alpha-5 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Integrin alpha-5 Polyclonal antibody

Integrin alpha-5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10569-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ITGA5, Integrin alpha-F, Integrin alpha-5 light chain, Integrin alpha-5 heavy chain, FNRA

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (33)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, 3T3-L1 cells, COS-7 cells, HeLa cells, human placenta tissue
Positive IHC detected in	human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 25 publications below
IHC	See 2 publications below
IF	See 7 publications below

Product Information

10569-1-AP targets Integrin alpha-5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity	human, mouse, monkey
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Integrin alpha-5 fusion protein Ag0860 相同性解析による交差性が予測される生物種
Full Name	integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Calculated molecular weight	115 kDa
Observed molecular weight	135-150 kDa
GenBank accession number	BC008786
Gene Symbol	Integrin alpha 5
Gene ID (NCBI)	3678
RRID	AB_2130060
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08648
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha-5 antibody 10569-1-AP	Download protocol
IHC protocol for Integrin alpha-5 antibody 10569-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Biomaterials Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment. Authors - Yonglin Yu View Article
mouse	WB	Int J Mol Sci Atp6v1h Deficiency Blocks Bone Loss in Simulated Microgravity Mice through the Fos-Jun-Src-Integrin Pathway Authors - Zanyan Zhao View Article
human	WB,IF	Front Cell Dev Biol Mesenchymal Stem Cells With Cancer-Associated Fibroblast-Like Phenotype Stimulate SDF-1/CXCR4 Axis to Enhance the Growth and Invasion of B-Cell Acute Lymphoblastic Leukemia Cells Through Cell-to-Cell Communication. Authors - Chengyun Pan View Article
human	WB	Oncotarget Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma. Authors - Huimin Wang View Article
		Sci Rep Evidence of novel miR-34a-based therapeutic approaches for multiple myeloma treatment. Authors - Mayra Rachele Zarone View Article
human	IF	Front Oncol ITGA5 Is a Novel Oncogenic Biomarker and Correlates With Tumor Immune Microenvironment in Gliomas. Authors - Shuyu Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10569-1-AP

WB analysis using 10569-1-AP

WB analysis of HeLa using 10569-1-AP

WB analysis of A549 using 10569-1-AP

WB analysis of human placenta using 10569-1-AP

WB analysis of COS-7 using 10569-1-AP

WB analysis of 3T3-L1 using 10569-1-AP

IHC staining of human skin cancer using 10569-1-AP

Integrin alpha-5 Polyclonal antibody

Integrin alpha-5 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.

Atp6v1h Deficiency Blocks Bone Loss in Simulated Microgravity Mice through the Fos-Jun-Src-Integrin Pathway

Mesenchymal Stem Cells With Cancer-Associated Fibroblast-Like Phenotype Stimulate SDF-1/CXCR4 Axis to Enhance the Growth and Invasion of B-Cell Acute Lymphoblastic Leukemia Cells Through Cell-to-Cell Communication.

Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma.

Evidence of novel miR-34a-based therapeutic approaches for multiple myeloma treatment.

ITGA5 Is a Novel Oncogenic Biomarker and Correlates With Tumor Immune Microenvironment in Gliomas.