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  • Western Blot (WB) analysis of HL-60 cells using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of HL-60 using 60258-1-Ig

    Untreated and PNGase F-treated lysates of HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Western Blot (WB) analysis of human spleen tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of human spleen using 60258-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of THP-1 using 60258-1-Ig

    Untreated and PNGase F-treated lysates of THP-1 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of THP-1 using 60258-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of HL-60 using 60258-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    WB analysis of U-937 using 60258-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human ovary cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human liver tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human ovary cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD11c Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human lung cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human breast cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human breast cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IHC staining of human liver cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IF Staining of human tonsillitis using 60258-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IF Staining of human tonsillitis using 60258-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IF Staining of human appendicitis using 60258-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CD11c/Integrin Alpha X Monoclonal antibody (60258-1-Ig)

    IF Staining of human appendicitis using 60258-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CD11c Monoclonal antibody

CD11c Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60258-1-Ig
Clone No.2F1C10
Publications(33)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, ELISA

Synonyms

2F1C10, CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman spleen tissue, THP-1 cells, HL-60 cells, U-937 cells
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human tonsillitis tissue, human liver tissue, human ovary cancer tissue, human lung cancer tissue, human appendicitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, human appendicitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60258-1-Ig targets CD11c in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11350

Product name: Recombinant human CD11c/Integrin alpha X protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-352 aa of BC038237

Sequence: NLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMA

相同性解析による交差性が予測される生物種
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight 1169 aa, 129 kDa
Observed molecular weight 145 kDa
GenBank accession numberBC038237
Gene Symbol CD11c
Gene ID (NCBI) 3687
ENSEMBL Gene IDENSG00000140678
RRIDAB_2881379
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP20702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Protocols

Product Specific Protocols
IF protocol for CD11c antibody 60258-1-IgDownload protocol
IHC protocol for CD11c antibody 60258-1-IgDownload protocol
WB protocol for CD11c antibody 60258-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cell

Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast cancer.

Authors - Rodrigo Nalio Ramos
View Article
humanIF

Sci Immunol

Initiator cell death event induced by SARS-CoV-2 in the human airway epithelium

Authors - Kaixin Liang
View Article

MedComm (2020)

Plasmacytoid and CD141+ Myeloid Dendritic Cells Cooperation with CD8+ T Cells in Lymph Nodes is Associated with HIV Control

Authors - Joana Vitallé
View Article
humanIHC

Med

CCL19+ dendritic cells potentiate clinical benefit of anti-PD-(L)1 immunotherapy in triple-negative breast cancer

Authors - Song-Yang Wu
View Article
humanIF

J Exp Med

AIM2 regulates anti-tumor immunity and is a viable therapeutic target for melanoma.

Authors - Keitaro Fukuda
View Article
humanIF

J Exp Med

PMID: 32181805

Authors - Xiaoyan Zhan
View Article
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