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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ITGB1BP1 Polyclonal antibody (12300-1-AP)

    WB analysis of PC-3 using 12300-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ITGB1BP1 Polyclonal antibody (12300-1-AP)

    WB analysis of human brain using 12300-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using ITGB1BP1 Polyclonal antibody (12300-1-AP)

    WB analysis of mouse thymus using 12300-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse thymus tissue using ITGB1BP1 Polyclonal antibody (12300-1-AP)

    IP experiment of mouse thymus using 12300-1-AP

    IP result of anti-ITGB1BP1 (IP:12300-1-AP, 4ug; Detection:12300-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using ITGB1BP1 Polyclonal antibody (12300-1-AP)

    IHC staining of human heart using 12300-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ITGB1BP1 Polyclonal antibody (12300-1-AP)

    IHC staining of human heart using 12300-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using ITGB1BP1 Polyclonal antibody (12300-1-AP)

    IHC staining of human colon using 12300-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using ITGB1BP1 Polyclonal antibody (12300-1-AP)

    IHC staining of human colon using 12300-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ITGB1BP1 Polyclonal antibody (12300-1-AP)

    IF Staining of PC-3 using 12300-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ITGB1BP1 antibody (12300-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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ITGB1BP1 Polyclonal antibody

ITGB1BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12300-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Integrin cytoplasmic domain-associated protein 1, Integrin beta-1-binding protein 1, ICAP-1, ICAP1, ICAP 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, mouse thymus tissue, human brain tissue
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman heart tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12300-1-AP targets ITGB1BP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITGB1BP1 fusion protein Ag2945 相同性解析による交差性が予測される生物種
Full Name integrin beta 1 binding protein 1
Calculated molecular weight 200 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC012264
Gene Symbol ITGB1BP1
Gene ID (NCBI) 9270
RRIDAB_10640896
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14713
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITGB1BP1, also named as ICAP1, is a key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail. It plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. ITGB1BP1 promotes transcriptional activity of the MYC promoter.

Protocols

Product Specific Protocols
WB protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
IHC protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
IF protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
IP protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols