CD61 / Integrin beta 3 Polyclonal antibody
CD61 / Integrin beta 3 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 18309-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Human peripheral blood platelets, mouse spleen tissue, rat spleen tissue |
Positive IP detected in | mouse spleen tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 36 publications below |
IHC | See 2 publications below |
IF | See 7 publications below |
IP | See 2 publications below |
FC | See 1 publications below |
CoIP | See 1 publications below |
Product Information
18309-1-AP targets CD61 / Integrin beta 3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, hamster |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) |
Calculated molecular weight | 87 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC127667 |
Gene symbol | Integrin beta 3 |
Gene ID (NCBI) | 3690 |
RRID | AB_2128759 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for CD61 / Integrin beta 3 antibody 18309-1-AP | Download protocol |
IP protocol for CD61 / Integrin beta 3 antibody 18309-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomaterials Construction and evaluation of novel αvβ3 integrin ligand-conjugated ultrasmall star polymer micelles targeted glomerular podocytes through GFB permeation. | ||
Theranostics A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance. | ||
Thromb Res Repurposing of rilpivirine for preventing platelet β3 integrin-dependent thrombosis by targeting c-Src active autophosphorylation | ||
NPJ Regen Med hUC-MSCs-derived MFGE8 ameliorates locomotor dysfunction via inhibition of ITGB3/ NF-κB signaling in an NMO mouse model |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sathyanarayani (Verified Customer) (04-05-2023) | I would highly recommend this primary antibody if you are looking for ITGB3. It produced really clear strong bands at 100kDa MW when used for western blot.
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FH Sathyanarayani (Verified Customer) (04-05-2023) | The ITGB3 antibody produced really nice clear bands on the western blot at 110Kda MW. I would highly recommend using this if you are measuring ITGB3.
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