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- KD/KO Validated
Centromere protein R Polyclonal antibody
Centromere protein R Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10743-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
Product Information
10743-1-AP targets Centromere protein R in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Centromere protein R fusion protein Ag1204 相同性解析による交差性が予測される生物種 |
| Full Name | integrin beta 3 binding protein (beta3-endonexin) |
| Calculated molecular weight | 20 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC009929 |
| Gene symbol | ITGB3BP |
| Gene ID (NCBI) | 23421 |
| RRID | AB_2128916 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ITGB3BP, also named as CENPR and NRIF3; is a transcription coregulator that can have both coactivator and corepressor functions. ITGB3BP acts as a coactivator for estrogen receptor alpha. It acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. ITGB3BP induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. ITGB3BP has five isoforms with MW 7 kDa, 13 kDa and 19-25 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Centromere protein R antibody 10743-1-AP | Download protocol |
| IHC protocol for Centromere protein R antibody 10743-1-AP | Download protocol |
| IF protocol for Centromere protein R antibody 10743-1-AP | Download protocol |
| IP protocol for Centromere protein R antibody 10743-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res Identification of a novel pathway that selectively modulates apoptosis of breast cancer cells. | ||
J Mol Cell Biol Phosphorylation of CENP-R by Aurora B regulates kinetochore-microtubule attachment for accurate chromosome segregation
| ||
Oncogene Serine 28 phosphorylation of NRIF3 confers its co-activator function for estrogen receptor-alpha transactivation. |
