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Species Reactivity

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  • Featured Product
  • KD/KO Validated

Integrin Beta 4 Polyclonal antibody

Integrin Beta 4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21738-1-AP

Synonyms

ITGB4, integrin, beta 4, Integrin beta-4, GP150, CD104

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    WB analysis using 21738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    WB analysis of A431 using 21738-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    WB analysis of A549 using 21738-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IP experiment of HeLa using 21738-1-AP

    IP result of anti-Integrin Beta 4 (IP:21738-1-AP, 4ug; Detection:21738-1-AP 1:500) with HeLa cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of mouse colon using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of mouse colon using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human placenta using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human placenta using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human pancreas cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human pancreas cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human breast cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human breast cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human pancreas cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IHC staining of human pancreas cancer using 21738-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Integrin Beta 4 Polyclonal antibody (21738-1-AP)

    IF Staining of A431 using 21738-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 4 antibody (21738-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inBxPC-3 cells, A431 cells, A549 cells, HT-29 cells, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse colon tissue, human placenta tissue, human pancreas cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21738-1-AP targets Integrin Beta 4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Beta 4 fusion protein Ag16450 相同性解析による交差性が予測される生物種
Full Name integrin, beta 4
Calculated molecular weight 1752 aa, 195 kDa
Observed molecular weight 200-240 kDa
GenBank accession numberBC126411
Gene symbol Integrin beta 4
Gene ID (NCBI) 3691
RRIDAB_10733888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 4 antibody 21738-1-APDownload protocol
IHC protocol for Integrin Beta 4 antibody 21738-1-APDownload protocol
IF protocol for Integrin Beta 4 antibody 21738-1-APDownload protocol
IP protocol for Integrin Beta 4 antibody 21738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,CoIP

Oncogene

Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.

Authors - Shaojia Wang
  • KD Validated
View Article
ratWB

Phytomedicine

Ganoderma lucidum polysaccharide inhibits HSC activation and liver fibrosis via targeting inflammation, apoptosis, cell cycle, and ECM-receptor interaction mediated by TGF-β/Smad signaling

Authors - Chaojie Chen
View Article
humanWB,IHC,IF

Sci Rep

Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma.

Authors - Xiao-Long Li
View Article
humanWB

Front Oncol

p63 Directs Subtype-Specific Gene Expression in HPV+ Head and Neck Squamous Cell Carcinoma.

Authors - Alexandra Ruth Glathar
View Article
humanWB,IHC

Cell Commun Signal

METTL14-mediated N6-methyladenosine modification of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma.

Authors - Zhuonan Liu
  • KD Validated
View Article
humanIF

Int J Oncol

FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.

Authors - Junfen Xu
View Article