Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, human lung tissue, K-562 cells, mouse spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
11328-1-AP targets Integrin Beta 7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Integrin Beta 7 fusion protein Ag1871 相同性解析による交差性が予測される生物種 |
| Full Name | integrin, beta 7 |
| Calculated molecular weight | 798 aa, 87 kDa |
| Observed molecular weight | 87-100 kDa |
| GenBank accession number | BC015916 |
| Gene Symbol | Integrin beta 7 |
| Gene ID (NCBI) | 3695 |
| RRID | AB_2249380 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26010 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ITGB7 (integrin, beta7) is a single-pass type I membrane protein that belongs to the integrin beta chain family. The integrins are a large family of cell-surface glycoprotein receptors that play key roles in leucocyte adhesion, signalling, proliferation and migration by binding as heterodimers to specific ligands. The beta7 integrin associates with alpha4 integrin to form a heterodimer, alpha4/beta7, which interacts with MADCAM1, VCAM, fibronectin, and may also interact with HIV-1 gp120. The beta7 integrin can also form a heterodimer with alphaE integrin, and the dimer, alphaE/beta7 integrin, is a receptor for E-cadherin. ITGB7 is expressed in a variety of leukocyte lines.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Beta 7 antibody 11328-1-AP | Download protocol |
| IHC protocol for Integrin Beta 7 antibody 11328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Reprod Expression of adhesion, attachment and invasion markers in eutopic and ectopic endometrium: a link to the aetiology of endometriosis. | ||
J Biol Chem Multifunctional activity of a small tellurium redox immunomodulator compound, AS101, on DSS-induced murine colitis. | ||
Cell Commun Signal Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis. | ||
Cell Mol Gastroenterol Hepatol Cholinergic anti-inflammatory pathway attenuates Acute Liver Failure through Inhibiting MAdCAM1/α4β7 -Mediated Gut-derived proinflammatory lymphocytes accumulation | ||
Arthritis Res Ther Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression | ||
J Ethnopharmacol Dan'e fukang decoction reduces hemorrhage in a rat model of mifepristone induced incomplete abortion and may correlate with cell adhesion molecule signaling interference |