Validation Data Gallery

Filter: AllWBIHC

WB analysis of human plasma using 24069-1-AP
human plasma were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 24069-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 24069-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of human hepatocirrhosis using 24069-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human hepatocirrhosis using 24069-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 24069-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 24069-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 40x lens).

ITIH4 Polyclonal antibody

ITIH4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24069-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Inter-alpha-trypsin inhibitor heavy chain H4, Inter-alpha-trypsin inhibitor family heavy chain-related protein, Inter-alpha-inhibitor heavy chain 4, IHRP, 70 kDa inter-alpha-trypsin inhibitor heavy chain H4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human plasma, mouse lung tissue, mouse serum, rat serum
Positive IHC detected in	human hepatocirrhosis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 4 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

24069-1-AP targets ITIH4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ITIH4 fusion protein Ag21275 相同性解析による交差性が予測される生物種
Full Name	inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
Calculated molecular weight	103 kDa
Observed molecular weight	120 kDa
GenBank accession number	BC136393
Gene Symbol	ITIH4
Gene ID (NCBI)	3700
RRID	AB_2879426
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q14624
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ITIH4 antibody 24069-1-AP	Download protocol
IHC protocol for ITIH4 antibody 24069-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cardiovasc Res Translatome profiling reveals Itih4 as a novel smooth muscle cell-specific gene in atherosclerosis Authors - Aarthi Ravindran View Article
human	IF	Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. Authors - Emna Ouni View Article
human	IHC	Front Oncol Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma. Authors - Changhong Yang View Article
mouse	WB	Clin Exp Immunol Citrullinated inter-alpha-trypsin inhibitor heavy chain 4 in arthritic joints and its potential effect in the neutrophil migration. Authors - Atsumu Osada View Article
	WB,IHC	BMC Cancer Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study. Authors - Naohiko Nakamura View Article
human	IHC	Dis Markers Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer. Authors - Xiao Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human plasma using 24069-1-AP

WB analysis of mouse lung using 24069-1-AP

WB analysis using 24069-1-AP

IHC staining of human hepatocirrhosis using 24069-1-AP

IHC staining of human hepatocirrhosis using 24069-1-AP

IHC staining of human liver using 24069-1-AP

IHC staining of human liver using 24069-1-AP

ITIH4 Polyclonal antibody

ITIH4 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Translatome profiling reveals Itih4 as a novel smooth muscle cell-specific gene in atherosclerosis

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.

Citrullinated inter-alpha-trypsin inhibitor heavy chain 4 in arthritic joints and its potential effect in the neutrophil migration.

Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study.

Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer.