ITIH4 Polyclonal antibody

ITIH4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA, IF

Conjugate

Unconjugated

Cat no : 24069-1-AP

Synonyms

Gp120, H4P, IHRP, ITI HC4, ITI heavy chain H4, ITIH4, ITIHL1, PK 120, PK120


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Tested Applications

Positive WB detected inhuman plasma, mouse lung tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24069-1-AP is ITIH4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITIH4 fusion protein Ag21275 相同性解析による交差性が予測される生物種
Full Name inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
Calculated molecular weight103 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC136393
Gene symbol ITIH4
Gene ID (NCBI) 3700
RRIDAB_2879426
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ITIH4 antibody 24069-1-APDownload protocol
IHC protocol for ITIH4 antibody 24069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cardiovasc Res

Translatome profiling reveals Itih4 as a novel smooth muscle cell-specific gene in atherosclerosis

Authors - Aarthi Ravindran
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
humanIHC

Front Oncol

Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.

Authors - Changhong Yang
mouseWB

Clin Exp Immunol

Citrullinated inter-alpha-trypsin inhibitor heavy chain 4 in arthritic joints and its potential effect in the neutrophil migration.

Authors - Atsumu Osada
WB,IHC

BMC Cancer

Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study.

Authors - Naohiko Nakamura
humanIHC

Dis Markers

Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer.

Authors - Xiao Jiang