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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ITK Monoclonal antibody, PBS Only

ITK Monoclonal Antibody for WB, IF, FC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9D8

Cat no : 67703-1-PBS

Synonyms

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

Validation Data Gallery

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  • Western Blot (WB) analysis of PBMC using ITK Monoclonal antibody (67703-1-Ig)

    WB analysis of PBMC using 67703-1-Ig (same clone as 67703-1-PBS)

    PBMC cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ITK Monoclonal antibody (67703-1-Ig)

    WB analysis of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ITK Monoclonal antibody (67703-1-Ig)

    IF Staining of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ITK antibody (67703-1-Ig, Clone: 1F9D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using ITK Monoclonal antibody (67703-1-Ig)

    FC experiment of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human ITK (67703-1-Ig, Clone:1F9D8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 90% MeOH . This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67703-1-PBS targets ITK in WB, IF, FC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ITK fusion protein Ag17087 相同性解析による交差性が予測される生物種
Full Name IL2-inducible T-cell kinase
Calculated molecular weight 620 aa, 72 kDa
Observed molecular weight70 kDa
GenBank accession numberBC109077
Gene symbol ITK
Gene ID (NCBI) 3702
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ITK is a member of Tec family (BTK, ITK, Tec, BMX and RLK), which expressed in normal T-lymphocytes and T-cell associated hematopoietic malignancies and have confirmed its critical role in regulating T lymphocyte function in EBV-driven lymphoproliferative disease and immune-mediated disorders. Tec kinase family members shares similarities structure, consisting of PH domain, SH3 domain, SH2 domain and kinase domain. Loss of function mutations in ITK results in hypogammaglobulinemia and CD4+ T cell loss in humans, and the patients often present with EBV-associated B cell lymphoproliferative syndrome (PMID: 30814910, PMID: 31025232).