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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ITLN2 Polyclonal antibody (18815-1-AP)

    WB analysis using 18815-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18815-1-AP (ITLN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ITLN2 Polyclonal antibody (18815-1-AP)

    IHC staining of human colon cancer using 18815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 18815-1-AP (ITLN2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ITLN2 Polyclonal antibody

ITLN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18815-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Intelectin-2, intelectin 2, HL2, HL 2, Endothelial lectin HL-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse small intestine tissue, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18815-1-AP targets ITLN2 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name intelectin 2
Calculated molecular weight 36 kDa
Observed molecular weight36 kDa
GenBank accession numberBC143341
Gene Symbol ITLN2
Gene ID (NCBI) 142683
RRIDAB_3669302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWU7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITLN2 also named as UNQ2789 and PRO7179, may play a role in the defense system against pathogens. Intelectins (intestinal lectins) are highly conserved across chordate evolution and have been implicated in various human diseases, including Crohn's disease (CD). The human genome encodes two intelectin genes, intelectin-1 (ITLN1) and intelectin-2 (ITLN2). It is reproted that human ITLN2 is expressed in Paneth cells, where it localizes to secretory granules, and establish that ITLN2 is a highly abundant multimeric protein secreted into the lumen of the small intestine (PMID: 35182405). The calculated molecular weight of ITLN2 is 36 kDa.

Protocols

Product Specific Protocols
WB protocol for ITLN2 antibody 18815-1-APDownload protocol
IHC protocol for ITLN2 antibody 18815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols