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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ITPK1 Polyclonal antibody

ITPK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10568-1-AP

Synonyms

Ins(1,3,4)P(3) 5/6 kinase, ITPK1, ITRPK1

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ITPK1 Polyclonal antibody (10568-1-AP)

    WB analysis of human brain using 10568-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ITPK1 Polyclonal antibody (10568-1-AP)

    WB analysis of HEK-293 using 10568-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using ITPK1 Polyclonal antibody (10568-1-AP)

    WB analysis of mouse eye using 10568-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using ITPK1 Polyclonal antibody (10568-1-AP)

    IHC staining of human brain using 10568-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10568-1-AP (ITPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using ITPK1 Polyclonal antibody (10568-1-AP)

    IHC staining of human brain using 10568-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10568-1-AP (ITPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, mouse eye tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10568-1-AP targets ITPK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITPK1 fusion protein Ag0834 相同性解析による交差性が予測される生物種
Full Name inositol 1,3,4-triphosphate 5/6 kinase
Calculated molecular weight 48 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC007428
Gene symbol ITPK1
Gene ID (NCBI) 3705
RRIDAB_2129832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ITPK1 antibody 10568-1-APDownload protocol
IHC protocol for ITPK1 antibody 10568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols