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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ITPKC Polyclonal antibody

ITPKC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14267-1-AP

Synonyms

IP3 3 kinase C, InsP 3-kinase C, InsP 3 kinase C, Inositol-trisphosphate 3-kinase C, Inositol 1,4,5-trisphosphate 3-kinase C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ITPKC Polyclonal antibody (14267-1-AP)

    WB analysis using 14267-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ITPKC Polyclonal antibody (14267-1-AP)

    WB analysis of human heart using 14267-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ITPKC Polyclonal antibody (14267-1-AP)

    IP experiment of HepG2 using 14267-1-AP

    IP result of anti-ITPKC (IP:14267-1-AP, 4ug; Detection:14267-1-AP 1:400) with HepG2 cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human breast cancer using 14267-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human breast cancer using 14267-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human heart using 14267-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human lymphoma using 14267-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human lymphoma using 14267-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ITPKC Polyclonal antibody (14267-1-AP)

    IHC staining of human heart using 14267-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ITPKC Polyclonal antibody (14267-1-AP)

    IF Staining of HepG2 using 14267-1-AP

    Immunofluorescent analysis of HepG2 cells, using ITPKC antibody 14267-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inMDA-MB-453s cells, human heart tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, human heart tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14267-1-AP targets ITPKC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITPKC fusion protein Ag5583 相同性解析による交差性が予測される生物種
Full Name inositol 1,4,5-trisphosphate 3-kinase C
Calculated molecular weight 75 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC060788
Gene symbol ITPKC
Gene ID (NCBI) 80271
RRIDAB_2280728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITPKC(Inositol-trisphosphate 3-kinase C) is also named as IP3KC and belongs to the inositol phosphokinase (IPK) family. It can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate and is relatively weakly activated by the calcium-calmodulin complex. ITPKC also acts as a negative regulator of T-cell activation through the Ca(2+)/NFAT signaling pathway, and the C allele may contribute to immune hyperreactivity in Kawasaki disease(PMID:17160344).

Protocols

Product Specific Protocols
WB protocol for ITPKC antibody 14267-1-APDownload protocol
IHC protocol for ITPKC antibody 14267-1-APDownload protocol
IF protocol for ITPKC antibody 14267-1-APDownload protocol
IP protocol for ITPKC antibody 14267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols