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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Intersectin 1 Polyclonal antibody

Intersectin 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21862-1-AP

Synonyms

ITSN1, SH3 domain-containing protein 1A, ITSN 1, ITSN, Intersectin-1

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using Intersectin 1 Polyclonal antibody (21862-1-AP)

    WB analysis of fetal human brain using 21862-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21862-1-AP (Intersectin 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Intersectin 1 Polyclonal antibody (21862-1-AP)

    IHC staining of human stomach using 21862-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using Intersectin 1 Polyclonal antibody (21862-1-AP)

    IHC staining of human stomach using 21862-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Intersectin 1 Polyclonal antibody (21862-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 21862-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Intersectin 1 Polyclonal antibody (21862-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 21862-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected infetal human brain tissue
Positive IHC detected inhuman stomach tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21862-1-AP targets Intersectin 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Intersectin 1 fusion protein Ag16517 相同性解析による交差性が予測される生物種
Full Name intersectin 1 (SH3 domain protein)
Calculated molecular weight 1721 aa, 195 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC116186
Gene symbol Intersectin 1
Gene ID (NCBI) 6453
RRIDAB_2878931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intersectin 1 (ITSN1) is a multidomain adaptor protein that plays a crucial role in various cellular processes, particularly in the nervous system. It is involved in clathrin-mediated endocytosis, signal transduction, and the regulation of the actin cytoskeleton. The protein is highly abundant in neurons and has been implicated in neurodevelopmental disorders, such as autism spectrum disorders, intellectual disability, and epilepsy. Recent studies have identified de novo variants in the ITSN1 gene in patients with these disorders, suggesting a link between ITSN1 and brain development.

Protocols

Product Specific Protocols
WB protocol for Intersectin 1 antibody 21862-1-APDownload protocol
IHC protocol for Intersectin 1 antibody 21862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Liquid Biopsy of Extracellular Vesicle-Derived miR-193a-5p in Colorectal Cancer and Discovery of Its Tumor-Suppressor Functions.

Authors - Rui Wei
View Article