Validation Data Gallery
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Tested Applications
| Positive WB detected in | Raji cells, NIH/3T3 cells |
| Positive IHC detected in | human skin cancer tissue, human thymus tissue, human oesophagus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 4 publications below |
| IF | See 6 publications below |
Product Information
55328-1-AP targets Involucrin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | involucrin |
| Calculated molecular weight | 69 kDa |
| Observed molecular weight | 80-90 kDa, 56-70 kDa |
| GenBank accession number | NM_005547 |
| Gene Symbol | Involucrin |
| Gene ID (NCBI) | 3713 |
| RRID | AB_11182270 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07476 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Involucrin antibody 55328-1-AP | Download protocol |
| IHC protocol for Involucrin antibody 55328-1-AP | Download protocol |
| IF protocol for Involucrin antibody 55328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis. | ||
J Ethnopharmacol The Water Extracts from the Oil Cakes of Prinsepia utilis Repair the Epidermal Barrier via Up-Regulating Corneocyte Envelope-Proteins, Lipid Synthases, and Tight Junction Proteins | ||
J Eur Acad Dermatol Venereol Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi. | ||
Oncotarget Transcriptional suppression of microRNA-27a contributes to laryngeal cancer differentiation via GSK-3β-involved Wnt/β-catenin pathway. | ||
Front Oncol Cystatin-B Negatively Regulates the Malignant Characteristics of Oral Squamous Cell Carcinoma Possibly Via the Epithelium Proliferation/Differentiation Program | ||
Int J Mol Sci Water-Soluble Fullerenol C60(OH)36 toward Effective Anti-Air Pollution Induced by Urban Particulate Matter in HaCaT Cell. |