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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IZUMO1 Polyclonal antibody

IZUMO1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 13296-1-AP

Synonyms

Sperm-specific protein izumo, OBF, Izumo sperm-egg fusion protein 1, IZUMO

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using IZUMO1 Polyclonal antibody (13296-1-AP)

    WB analysis of mouse testis using 13296-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13296-1-AP (IZUMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using IZUMO1 Polyclonal antibody (13296-1-AP)

    IHC staining of human testis using 13296-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13296-1-AP (IZUMO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using IZUMO1 Polyclonal antibody (13296-1-AP)

    IHC staining of human testis using 13296-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13296-1-AP (IZUMO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using IZUMO1 Polyclonal antibody (13296-1-AP)

    IF Staining of mouse testis using 13296-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 13296-1-AP (IZUMO1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13296-1-AP targets IZUMO1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IZUMO1 fusion protein Ag4008 相同性解析による交差性が予測される生物種
Full Name izumo sperm-egg fusion 1
Calculated molecular weight 350 aa, 39 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC034769
Gene symbol IZUMO1
Gene ID (NCBI) 284359
RRIDAB_2877934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IZUMO1, also named as OBF, is a sperm-specific protein. It is a novel member of the immunoglobulin superfamily located on sperm and is indispensable for sperm-egg fusion. For mouse Izumo1, the MW is about 56 kDa. For human IZUMO1, the MW is about 37-40 kDa in WB detection.

Protocols

Product Specific Protocols
WB protocol for IZUMO1 antibody 13296-1-APDownload protocol
IHC protocol for IZUMO1 antibody 13296-1-APDownload protocol
IF protocol for IZUMO1 antibody 13296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols