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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Icln Polyclonal antibody

Icln Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25964-1-AP

Synonyms

CLCI, CLNS1A, CLNS1B, I(Cln), ICln, Methylosome subunit pICln, pICln, Reticulocyte pICln

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Icln Polyclonal antibody (25964-1-AP)

    WB analysis of HEK-293 using 25964-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25964-1-AP (Icln antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDCK cells using Icln Polyclonal antibody (25964-1-AP)

    WB analysis of MDCK using 25964-1-AP

    MDCK cells were subjected to SDS PAGE followed by western blot with 25964-1-AP (Icln antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Icln Polyclonal antibody (25964-1-AP)

    IF Staining of HEK-293 using 25964-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25964-1-AP (Icln antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, MDCK cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25964-1-AP is Icln Fusion Protein expressed in E. coli.

Tested Reactivity human, Canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Icln fusion protein Ag22923 相同性解析による交差性が予測される生物種
Full Name chloride channel, nucleotide-sensitive, 1A
Calculated molecular weight 237 aa, 26 kDa
Observed molecular weight 36-42 kDa
GenBank accession numberBC119634
Gene symbol CLNS1A
Gene ID (NCBI) 1207
RRIDAB_2880312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Icln antibody 25964-1-APDownload protocol
IF protocol for Icln antibody 25964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols