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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse IFN-gamma Rabbit Recombinant Antibody, PBS Only

IFN-gamma Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240262C9

Cat no : 98045-1-PBS

Synonyms

IFN gamma, Ifng, IFN γ, IFN g, Ifg

Validation Data Gallery

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  • Flow cytometry (FC) experiment of C57BL/6 Th1-polarized splenocytes using Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR)

    FC experiment of C57BL/6 Th1-polarized splenocytes using 98045-1-RR (same clone as 98045-1-PBS)

    1x10^6 unstimulated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with APC Anti-Mouse CD4 (GK1.5) and 0.25 ug Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR, Clone:240262C9) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98045-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of C57BL/6 Th1-polarized splenocytes using Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR)

    FC experiment of C57BL/6 Th1-polarized splenocytes using 98045-1-RR (same clone as 98045-1-PBS)

    1x10^6 PMA, Ionomycin and Brefeldin A treated(red) or untreated(blank) C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR, Clone:240262C9) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98045-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98045-1-RR

    Affinity and Kinetic Characterization of 98045-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98045-1-RR against Mouse IFN-gamma were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98045-1-PBS is IFN-gamma Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interferon gamma
Calculated molecular weight17 kDa
GenBank accession numberBC119063
Gene symbol IFN gamma
Gene ID (NCBI) 15978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.