Rabbit IgG Isotype Control Recombinant Antibody

IgG Isotype Control Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

WB, IHC, IF/ICC, FC, FC (Intra), IP

Conjugate

Unconjugated

CloneNo.

240953C9

Cat no : 98136-1-RR

Synonyms

Rabbit IgG Isotype Control, Rabbit IgG isotype, Rabbit IgG Control, Rabbit IgG


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Tested Applications

Positive WB detected in
Positive IP detected in
Positive IHC detected in
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Positive FC (Intra) detected in
Positive FC detected in

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98136-1-RR targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name IgG Isotype Control
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Protocols

Product Specific Protocols
WB protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IHC protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IF protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IP protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols