Validation Data Gallery

Filter: AllWBSDS-PAGEIPIHCIF/ICCFC (Intra)FC

WB analysis using 98136-1-RR
HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 98136-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
SDS-PAGE experiment of antibody using 98136-1-RR
Purity of 98136-1-RR (IgG Isotype Control antibody) was determined by SDS- polyacrylamide gel electrophoresis. The antibody was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
IP experiment of HepG2 using 98136-1-RR
IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug.
IHC staining of human placenta using 98136-1-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 82719-13-RR (PD-L1/CD274 Recombinant antibody) or unstained (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 98136-1-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 98136-1-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:2000 (right) or 84192-3-RR (KI67 Recombinant antibody) (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 98136-1-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:2000 (right) or 84192-3-RR (KI67 Recombinant antibody) (left) (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 98136-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control.
IF Staining of HeLa using 98136-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of MCF-7 using 98136-1-RR
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).PCYT1A Recombinant antibody (83100-1-RR) is a positive control.
IF Staining of K-562 using 98136-1-RR
Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).CD71 Recombinant antibody (81793-2-RR) is a positive control.
IF Staining of A549 using 98136-1-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).TMEM53 Recombinant antibody (83554-3-RR) is a positive control.
FC experiment of SH-SY5Y using 98136-1-RR
1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
×
1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of MCF-7 using 98136-1-RR
1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone:240159G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
×
1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone:240159G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of RAW 264.7 using 98136-1-RR
1x10^6 RAW 264.7 cells were intracellularlly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2ug IGFBP2 Recombinant antibody (84092-3-RR, Clone: 241363D8) and PE-Conjugated Goat Anti-Rabbit IgG (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
×
1x10^6 RAW 264.7 cells were intracellularlly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2ug IGFBP2 Recombinant antibody (84092-3-RR, Clone: 241363D8) and PE-Conjugated Goat Anti-Rabbit IgG (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of human PBMCs using 98136-1-RR
1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed.
FC experiment of rat splenocytes using 98136-1-RR
1x10^6 rat splenocytes cells were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.2 ug Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody (98060-1-RR, Clone:240725C2) and PE-conjugated Goat Anti-Rabbit IgG, or unstain. Cells were not fixed.
FC experiment of HeLa using 98136-1-RR
1x10^6 HeLa cells were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug Anti-Human MICA Rabbit Recombinant Antibody (98110-1-RR, Clone:240735D5) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were not fixed.
FC experiment of mouse splenocytes using 98136-1-RR
1x10^6 mouse splenocytes were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.2 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or unstain. Cells were not fixed.

Rabbit IgG Isotype Control Recombinant Antibody

IgG Isotype Control Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP

Cat No. 98136-1-RR

Clone No.240953C9

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

WB, IHC, IF/ICC, FC, FC (Intra), IP

Synonyms

Rabbit IgG Isotype Control, Rabbit IgG isotype, Rabbit IgG Control, Rabbit IgG

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

APC CoraLite® Plus 405 CoraLite® Plus 488 CoraLite® Plus 647 CoraLite® Plus 750 CoraLite®594 FITC Plus PE

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in
Positive IP detected in
Positive IHC detected in	**Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in
Positive FC (Intra) detected in
Positive FC detected in

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98136-1-RR targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP applications and shows reactivity with n/a samples.

Tested Reactivity	n/a
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	IgG Isotype Control
Gene Symbol
Gene ID (NCBI)
RRID	AB_3672282
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
Storage Buffer	PBS with 0.09% sodium azide , pH 7.3
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Protocols

Product Specific Protocols
WB protocol for IgG Isotype Control antibody 98136-1-RR	Download protocol
IHC protocol for IgG Isotype Control antibody 98136-1-RR	Download protocol
IF protocol for IgG Isotype Control antibody 98136-1-RR	Download protocol
IP protocol for IgG Isotype Control antibody 98136-1-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 98136-1-RR

WB analysis using 98136-1-RR

SDS-PAGE experiment of antibody using 98136-1-RR

IP experiment of HepG2 using 98136-1-RR

IHC staining of human placenta using 98136-1-RR

IHC staining of mouse colon using 98136-1-RR

IHC staining of human tonsillitis using 98136-1-RR

IHC staining of human tonsillitis using 98136-1-RR

IF Staining of HeLa using 98136-1-RR

IF Staining of HeLa using 98136-1-RR

IF Staining of MCF-7 using 98136-1-RR

IF Staining of K-562 using 98136-1-RR

IF Staining of A549 using 98136-1-RR

FC experiment of SH-SY5Y using 98136-1-RR

FC experiment of MCF-7 using 98136-1-RR

FC experiment of RAW 264.7 using 98136-1-RR

FC experiment of human PBMCs using 98136-1-RR

FC experiment of rat splenocytes using 98136-1-RR

FC experiment of HeLa using 98136-1-RR

FC experiment of mouse splenocytes using 98136-1-RR