CoraLite® Plus 488-conjugated Rabbit IgG control Polyclonal antibody
Rabbit IgG control Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
n/a
Applications
FC (Intra), IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-30000
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
CL488-30000 targets Rabbit IgG control in FC (Intra), IF applications and shows reactivity with n/a samples.
Tested Reactivity | n/a |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | N/A 相同性解析による交差性が予測される生物種 |
Full Name | Rabbit IgG control |
Gene symbol | |
Gene ID (NCBI) | |
RRID | AB_2919216 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | N/A |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 Rabbit IgG control antibody CL488-30000 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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