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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Human IgG Lambda Light Chain Polyclonal antibody

Human IgG Lambda Light Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 20758-1-AP

Synonyms

IgG Lambda Light Chain, Human IgG lambda chain, IGLC1, Immunoglobulin lambda constant 1, IGLC

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Human IgG Lambda Light Chain Polyclonal antibody (20758-1-AP)

    WB analysis of human plasma using 20758-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG Lambda Light Chain Polyclonal antibody (20758-1-AP)

    IHC staining of human tonsillitis using 20758-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG Lambda Light Chain Polyclonal antibody (20758-1-AP)

    IHC staining of human tonsillitis using 20758-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Human IgG Lambda Light Chain Polyclonal antibody (20758-1-AP)

    IF Staining of human tonsillitis using 20758-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG Lambda Light Chain antibody (20758-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20758-1-AP targets Human IgG Lambda Light Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Human IgG Lambda Light Chain fusion protein Ag14693 相同性解析による交差性が予測される生物種
Full Name immunoglobulin lambda constant 1 (Mcg marker)
Calculated molecular weight 233 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC007782
Gene symbol IgG Lambda Light Chain
Gene ID (NCBI) 3537
RRIDAB_2878734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the lambda light chain of human IgG.

Protocols

Product Specific Protocols
WB protocol for Human IgG Lambda Light Chain antibody 20758-1-APDownload protocol
IHC protocol for Human IgG Lambda Light Chain antibody 20758-1-APDownload protocol
IF protocol for Human IgG Lambda Light Chain antibody 20758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC,IF

iScience

SFTSV infection is associated with transient overproliferation of monoclonal lambda-type plasma cells

Authors - Chuansong Quan
View Article