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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse IgG1 Isotype Control (MOPC-21)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Applications

FC

Conjugate

Unconjugated

CloneNo.

MOPC-21

Cat no : 65124-1-Ig

Synonyms

IgG1, IgG1 Isotype Control, mouse IgG1, Mouse IgG1 Isotype Control

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig)

    FC experiment of human PBMCs using 65124-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Mouse IgG1 Isotype Control (65124-1-Ig, Clone:MOPC-21) (red) or 0.2ug Anti-Human CD8a (65113-1-Ig, Clone:Hit8a) (green) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000 or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65124-1-Ig targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant gamma 1 (G1m marker)
Gene symbol IgG1 Isotype Control
Gene ID (NCBI) 16017
RRIDAB_2918418
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for IgG1 Isotype Control antibody 65124-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols