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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Mouse IgG2a Isotype Control (MOPC-173) Recombinant (65571-1-MR)

    FC experiment of human PBMCs using 65571-1-MR (same clone as 65571-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.2 ug Mouse IgG2a Isotype Control (MOPC-173) Recombinant Antibody (65571-1-MR, Clone:MOPC-173) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG2a Isotype Control (MOPC-173) (65244-1-Ig, Clone: MOPC-173) (blue), or 0.2 ug Anti-Human CD8 (UCHT4) (65204-1-Ig, Clone: UCHT4)(green). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65571-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Mouse IgG2a Isotype Control (MOPC-173) Recombinant (65571-1-PBS)

    FC experiment of human PBMCs using 65571-1-PBS

    1x10^6 human PBMCs were surface stained with 0.2 ug Mouse IgG2a Isotype Control (MOPC-173) Recombinant Antibody, PBS Only (65571-1-PBS, Clone: MOPC-173) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.2 ug Mouse IgG2a Isotype Control (MOPC-173) (65244-1-Ig, Clone: MOPC-173) (blue), or 0.2 ug Anti-Human CD8 (UCHT4) (65204-1-Ig, Clone: UCHT4) (green). Cells were not fixed. Lymphocytes were gated.

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Mouse IgG2a Isotype Control (MOPC-173) Recombinant Antibody, PBS Only

IgG2a Isotype Control Uni-rAbTM Recombinant Antibody for FC
Cat No. 65571-1-PBS
Clone No.MOPC-173

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

n/a

Applications

FC

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65571-1-PBS targets IgG2a Isotype Control in FC applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Mouse / IgG2a, kappa
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name IgG2a Isotype Control
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for IgG2a Isotype Control antibody 65571-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols