Mouse IgM Isotype Control (MOPC-104E)

FC experiment of human whole blood using 65243-1-Ig

Human whole blood was treated with ACK lysis buffer then 1x10^6 cells were surface stained with 0.2 ug Mouse IgM Isotype Control (MOPC-104E) (65243-1-Ig) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) (red), or CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) alone (black dashed), or unstained (blue). Cells were not fixed. Lymphocytes were gated.