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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IkB Alpha Recombinant antibody, PBS Only

IkB Alpha Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C24

Cat no : 80019-1-PBS

Synonyms

IKBA, IκB α, NFKBIA, IkB-alpha, I-kappa-B-alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IkB Alpha Recombinant antibody (80019-1-RR)

    WB analysis using 80019-1-RR (same clone as 80019-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80019-1-RR (IkB alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IkB Alpha Recombinant antibody (80019-1-RR)

    IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IkB Alpha Recombinant antibody (80019-1-RR)

    IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IkB Alpha Recombinant antibody (80019-1-RR)

    IHC staining of human prostate cancer using 80019-1-RR (same clone as 80019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IkB Alpha Recombinant antibody (80019-1-RR)

    IHC staining of human prostate cancer using 80019-1-RR (same clone as 80019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80019-1-RR

    Affinity and Kinetic Characterization of 80019-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80019-1-RR against Human IkB Alpha were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80019-1-PBS targets IkB Alpha in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IkB Alpha fusion protein Ag0345 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated molecular weight 36 kDa
Observed molecular weight36 kDa and 35 kDa
GenBank accession numberBC002601
Gene symbol IkB Alpha
Gene ID (NCBI) 4792
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. IkB alpha can be observed as a band at 36 kDa in human cell lysates, and 35 kDa in mouse and rat cell ysates.