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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL3RA/CD123 Polyclonal antibody

IL3RA/CD123 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30012-1-AP

Synonyms

Il3ra, Sut-1, IL-3R-alpha, IL-3RA, IL-3R subunit alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Il3ra Polyclonal antibody (30012-1-AP)

    WB analysis using 30012-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30012-1-AP (Il3ra antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Il3ra Polyclonal antibody (30012-1-AP)

    WB analysis using 30012-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30012-1-AP (Il3ra antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using IL3RA/CD123 Polyclonal antibody (30012-1-AP)

    IF Staining of mouse brain using 30012-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Il3ra antibody (30012-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inPC-12 cells, NIH/3T3 cells
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30012-1-AP targets IL3RA/CD123 in WB, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL3RA/CD123 fusion protein Ag32002 相同性解析による交差性が予測される生物種
Full Name interleukin 3 receptor, alpha chain
Calculated molecular weight43 kDa
Observed molecular weight43 kDa, 60-70 kDa
GenBank accession numberNM_008369
Gene symbol Il3ra
Gene ID (NCBI) 16188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-3RA, also named as CD123 and SUT-1, is the alpha subunit of the interleukin 3 (IL-3) receptor, which is part of the type I cytokine receptor superfamily (PMID: 25043189). It can regulate the proliferation, survival, and differentiation of hematopoietic cells. In mouse, there are two classes of high-affinity IL-3 receptors. One contains an IL-3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL-5 and GM-CSF receptors. This antibody is specific for mouse Il3ra.

Protocols

Product Specific Protocols
WB protocol for IL3RA/CD123 antibody 30012-1-APDownload protocol
IF protocol for IL3RA/CD123 antibody 30012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols