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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Inhibin beta A-Specific Monoclonal antibody, PBS Only

Inhibin beta A-Specific Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F8D8

Cat no : 60352-1-PBS

Synonyms

Activin beta A chain, EDF, FRP, INHBA, INHBA-Specific, Inhibin beta A chain, Inhibin beta A-Specific, inhibin, beta A

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of HepG2 using 60352-1-Ig (same clone as 60352-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of human placenta using 60352-1-Ig (same clone as 60352-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (Inhibin beta A-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of pig liver using 60352-1-Ig (same clone as 60352-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (Inhibin beta A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of pig liver using 60352-1-Ig (same clone as 60352-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of human testis using 60352-1-Ig (same clone as 60352-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of L02 using 60352-1-Ig (same clone as 60352-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:650 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human lung cancer using 60352-1-Ig (same clone as 60352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human lung cancer using 60352-1-Ig (same clone as 60352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human oesophagus cancer using 60352-1-Ig (same clone as 60352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human oesophagus cancer using 60352-1-Ig (same clone as 60352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human stomach cancer using 60352-1-Ig (same clone as 60352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human stomach cancer using 60352-1-Ig (same clone as 60352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60352-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60352-1-PBS targets Inhibin beta A-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name inhibin, beta A
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberNM_002192
Gene symbol INHBA
Gene ID (NCBI) 3624
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Inhibin βA(INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.